Immobilized Thermomyces lanuginosus lipase for Methyl Ester Production from Sunflower Oil
Sustainable process-product development & green chemistry
Sustainable & Clean Technologies - IIa: Energy Production (T1-5a)
Keywords: Thermomyces lanuginosus lipase, biodiesel production, immobilization
Transesterification reaction was performed using triglycerides and alcohol by immobilized lipase in absence of aqueous conditions. The fatty acid methyl esters, which is the product of this reaction, can be used as a biodiesel that does not produce sulfur oxide and minimize the cancerogenic particulate such as poly aromatic hydrocarbons.
In this study, immobilized lipase from Thermomyces lanuginosus was studied to convert triglycerides to fatty acid methyl esters, which is commonly named as a biodiesel, using methanol. İmmobilization of lipase was carried out using polystyrene-divinylbenzene copolymer that includes aldehyde functional groups. It was used such as a micro porous polymeric enzyme reactor and a carrier. Thermomyces lanuginosus lipase was immobilized on the carrier with 48% immobilization yield. During enzymatic transesterification of sunflower oil with methanol for biodiesel production, the maximum biodiesel yield was 76% after 1 hour and 97% after 3 hours, in respectively. Organic solvent is not necessary in this reaction. Methanol was added in the reaction system at three steps. At the first step, alcohol was added 1/3 molar equivalent of MeOH for the stoichiometric amount and the second, third- step reaction was performed by adding 2/3 molar equivalent of MeOH. The immobilized lipase onto the STR-DVB copolymer proved to be stable after even 30 reused and lost little activity when was subjected to repeated use.
In the second study, copolymer was ground and brought very slender. Thermomyces lanuginosus lipase was immobilized with 44% immobilization yield. The maximum biodiesel yield was 69% after 1 hour and 95% after 3 hours, in respectively. The free glycerol and residual mono-, di- and triglyceride contents and ester contents of fatty acid methyl esters (FAME) was determined by DIN EN 14105 and DIN EN 14103 methods, respectively.
Presented Wednesday 19, 16:00 to 16:15, in session Sustainable & Clean Technologies - IIa: Energy Production (T1-5a).
