diet syrap as a new sugar source in fermentative lysine production
Special Symposium - Innovations in Food Technology (LMC Congress)
Innovations in Food Technology - Poster Session (LMC/Food - P1)
Keywords: Date syrup, Lysine, Corynebacterium Glutamicum, Brevibactrium Fluvum
Lysine is an essential, economically important amino acid used as food ?and feed supplement. It has also some pharmaceutical applications in the? formulation of diets with balanced amino acids composition and in amino ?acid infusions. Chemical, enzymatic and fermentation processes have ?been used to synthesize lysine. However, economical production of lysine depends on the availability of local resources. In Middle East countries, date is an ?important agricultural product which has the potential for being a nutritious source for lysine production. In this work, date syrup was selected as an alternative complex sugar source for lysine production. Brevibacterium flavium and Corynebacterium glutamicum were used to examine the effect of variation in culture composition and operating conditions on growth and lysine formation. Lysine production and bacterial growth were analyzed by acidic ninhydrin method ?of Chinard and optical density, respectively. Due to the problems in Lysine analysis caused by interference or date syrup colour, a decolourizing step was used before applying Ninhydrin Test. Results show that we have 3.4g/l lysine production at the maximum level.
