Date syrup alternative source for lysine production
Special Symposium - Innovations in Food Technology (LMC Congress)
White BioTech & Related Processes (Food-2b)
Keywords: lysine,date syrap, Brevibacterium flavum, Corynebacterium glutamicum
Lysine is an essential, economically important amino acid used as food and feed supplement. It has also some pharmaceutical applications in the formulation of diets with balanced amino acids composition and in amino acid infusions. Chemical, enzymatic and fermentation processes have been used to synthesize lysine.However, economical production of lysine depends on the availability of local resources. In Middle East countries, date is an important agricultural product which has the potential for being a nutritious source for lysine production.
In this work, date syrup was selected as an alternative complex sugar source for lysine production. Brevibacterium flavum and Corynebacterium glutamicum were used to examine the effect of variation in culture composition and operating conditions on growth and lysine formation. Lysine production and bacterial growth were analyzed by acidic ninhydrin method of Chinard and optical density, respectively.Due to the problems in lysine analysis caused by interference of date syrup colour, a decolourizing step was used before applying
ninhydrin test. Results showed a maximum of 3.4 g/l lysine production under the examined composition and conditions by Corynebactrium glutamicum.
Presented Wednesday 19, 17:05 to 17:10, in session White BioTech & Related Processes (Food-2b).
