CHARACTERIZATION OF A BIOSURFACTANT PRODUCED BY PSEUDOMONAS FLUORESCENS
Integration of life sciences & engineering
Integration of Life Sciences & Engineering - Poster (T5-P)
Keywords: Biosurfactant , charactérization, Pseudomonas, emulsification, stability
ABSTRACT
The production of a biosurfactant by Pseudomonas fluorescens Migula 1895-DSMZ was studied. Cultures conditions involving variations in carbon and nitrogen sources and different C:N ratois were examined at constant temperature and pH, with the aim of increasing productivity in the process . Biosurfactant synthesis was followed by measuring surface tension and emulsifying index E24 .
The best results were obtained when using olive oil and ammonium nitrate as carbon and nitrogen sources respectively with a C:N ratio of 10. The production of biosurfactant was growth associated as indicated by the growth and biosurfactant production kinetics . The surface tension was reduced to below 32 dyne/cm and with emulsification index E24 of 65% in 36 to 48 hours . The properties of biosurfactant that was separated by acetone precipitation were investigated . It showed stability during exposure to high temperatures (up to 120 °C for 15 minutes) , high salinity (5-10% Nacl) and a wide range of pH(4-9). The biosurfactant had a good foaming and emulsifying and antimicrobial activities . Analysis by biochemical test(rhamnose test) and infrared spectroscopy (FTIR) showed that the biosurfactant was a rhmnolipid-type in nature .
Presented Wednesday 19, 13:30 to 15:00, in session Integration of Life Sciences & Engineering - Poster (T5-P).
