Development of a Horseradish peroxidase-based Flow Injection Amperometric Biosensor for the Determination of Phenolic Compounds
Integration of life sciences & engineering
Integration of Life Sciences & Engineering - Poster (T5-P)
Keywords: Biosensor, horseradish peroxidase, phenol
An amperometric biosensor has been developed for the quantitative estimation of phenolics (catechol, phenol, p-benzoquinone) in aqueous solution. The enzyme, horseradish peroxidase was chemically immobilized on a polygluteraldehyde modified polypyrolle (PGA/PPy/HRP) polymer film, electrochemically prepared onto a glassy carbon electrode. The enzyme electrode system was constructed in a continuous flow cell. While a phosphate buffer solution (100 mM, pH 7) was passing through the system, various concentration of (0.0025-7 mM) phenol solutions with the volume of 1 mL were injected to this carrier solution by using an injection valve. Amperometric response was measured as a function of concentration of phenolic compound, at fixed voltage of -50mV. The electrode showed a wide linear range for the p-benzoquinone between 0.01-0.75 (r=0.997) mM . Optimum hydrogen peroxide concentration and pH for the system were found to be 1.5 mM and 7, respectively. The stability of the amperometric peaks was determined for the consecutive14 p-benzoquinone injections giving the best response with the standard deviation of ± 2.9×10-9 .
Presented Wednesday 19, 13:30 to 15:00, in session Integration of Life Sciences & Engineering - Poster (T5-P).
