DNA Nanobiosensor Based on Silicon Chip
Multi-scale and/or multi-disciplinary approach to process-product innovation
Nanotechnology & Nanomanufacturing (T3-1P)
Keywords: DNA,Nanobiosensor,Electrochemistry,Self assembly
Au nanoparticles heavily functionalized with oligonucleotides have been used in a variety of DNA detection methods. The electrochemical properties of DNA immobilized to Au Nanoparticle deposited onto suitable surfaces have been analyzed to detect hybridization processes of specific DNA sequences as possible alternatives to fluorescent labeling methods. This paper reports on the preparation of gold nanoparticles
directly deposited onto the surface of silicon (Si) substrate by a physical methodology, consisting in the thermal evaporation of a thin Au film and its successive annealing. in the next step self-assembly of thiolated DNA and mercaptohexanol spacer, as well as the DNA hybridization have been sensed by the redox signals of base residues in DNA immobilized at the surface of gold nanoparticles. Electrochemical biosensing of DNA hybridization is not only uniquely qualified for meeting the size, cost, and power requirements of decentralized genetic testing, but offer an elegant route for interfacing—at the molecular level—the DNA-recognition and signal-transduction elements. We show that the changes of electrochemical signals before and after DNA functionalization and subsequent hybridization of Au nanoparticles immobilized onto Si substrate can be exploited to fabricate specific nanobiosensors devices in solid phase.
Presented Tuesday 18, 13:30 to 15:00, in session Nanotechnology & Nanomanufacturing (T3-1P).
