Matthew B. Kerby1, Kristina Prachanronarong2, Tiffany Yizar2, Anubhav Tripathi2, Andrew W. Artenstein3, Steve M. Opal3, and Sarah Freeman2. (1) Brown University, Division of Engineering, Box D, Providence, RI 02912, (2) Engineering, Brown University, Division of Engineering, Box D, Providence, RI 02912, (3) Brown Medical School, Memorial Hospital of RI, 111 Brewster St., Pawtucket, RI 02860
The poster presents a study on isolation of target sequences, quantifying binding efficiency, and calculations of minimum viral load density needed in the samples of synthetic, non-virulent vRNA sequences for human hemagglutinin (H) and neuraminidase (N) genes. DNA primer/promoters are created to capturing the vRNA by linking to beads for purification. Purified vRNA is converted to cDNA and amplified in a continuous flow reaction using a T7-polymerase. The rates of RNA synthesis, durability, amplification efficiency are evaluated. The synthesized sequences are evaluated with specifically designed molecular beacon sequences identifying a known human influenza subtypes H1N1. The hybridization specificity, reaction time and signal to noise ratios are evaluated.