Jin Hyung Lee1, Byoung Chan Kim1, Jungbae Kim2, Byoung-In Sang3, and Man Bock Gu4. (1) Advanced Environmental Monitoring Research Center (ADEMRC), Gwangju Institute of Science and Technology (GIST), 1, Oryoung-dong, Puk-Gu, Gwangju, South Korea, (2) Pacific Northwest National Laboratory, 902 Battelle Blvd., PO Box 999, Mailstop K8-93, Richland, WA 99352, (3) Hazardous substances research center, Korea Institute of Science and Technology, Seoul, South Korea, (4) College of Life sciences and biotechnology, Korea University, 1Anam-dong, Seongbuk-gu, Seoul, South Korea
With increasing demand the stable enzyme immobilization system has become an essential for enzyme application in various fields. The use of electrospun nanofibers in enzyme immobilization, one of solution for the demand, is attractive to enzyme immobilization owing to low mass transfer limitation, ease of changing structure and simple to manufacture. Here, we show the application of immobilized enzymes on nanofiber to biodegradation process. Various enzyme-nanofiber systems were prepared by using different enzymes, for instance, different origins of esterase (Rhizopus oryzae, Bacillus thermoglucosidsius and Saccharomyces cerevisiae), lignin peroxidase (LiP) and manganese peroxidase (MnP). We tested activity and stability of the enzymes and compared its biocatalytic capabilities. These biocatalytic systems were also applied to remove environmental pollutants via biodegradation process. This study will show the practical application of enzyme immobilized nanofiber systems in biodegradation and bioremediation.