Mesoporous carbons with well defined large-sized pores and intrinsic electrical conductivity are a good candidate as a host of enzyme molecules for bioelectrochemical applications. In this contribution, mesoporous carbons were used for the immobilization of a redox enzyme. We report the unique characteristics of mesoporous carbons and their use as a host matrix for enzyme immobilization and further applications in glucose sensing, magnetically switchable bio-electrocatalytic system, and biofeul cells.