We advocated "Origami" that is a concept to design the process for constructing organs. It is the concept to think about the combination of the most efficient unit processes by understanding the structure of organs, and folding out the organs. Therefore, it can be said, "Origami" is "Retrosynthesis of organs". In the present study, we showed that complex three-dimensional cell patterning was able in the combination of transferring printing of the cell using cell-surface modifier and in situ gelation to make extracellar matrix sheet using transglutaminase. In the use of the cell patterning technology developed, the line pattern of cells which width was 50 μm was printed on the extracellular matrix sheet. The model structure that diverged radially from the center like an internal structure of lungs was constructed. At first, we focused on the diverged structure of the target and was extracted some features. We found that the structure was regularly folded two-dimensional sheet, and unfolded the structure to two-dimensional sheet. The extracellular matrix sheet on which the cell pattern was printed was folded to the reverse order of this unfolding process. As a result, the target structure was able to be constructed efficiently. The designing of the process to construct organs by the "Origami" concept showed that three-dimensional patterning of cells was able to be done more efficiently than a previous method.