Glucose levels correspond to brain metabolic activity, so observation of glucose concentration is a possible window into how memories are formed. In the laboratory a microdialysis unit is used to determine the glucose levels in a rat's hippocampus. The standard method involves running dialysate through the system for one hour before collecting the perfusate to analyze its composition. The underlying assumption is that mass transfer achieves steady state after one hour. The purpose of this study was to determine if one hour was sufficient for the system to come to steady-state. Additionally, the accuracy of the Zero Net Flux method of concentration determination was analyzed. FEMLAB computer software was used to simulate the dynamics of microdialysis. Though the relevant physical constants for the system were given, several of the values were estimates rather than known values, so they were varied and their effect on steady-state time and the concentration profile was observed. The variables investigated included the dialysate inlet concentration, the effective diffusivity through brain tissue, the distance from the needle to the glucose source, and the dialysate volume flow rate. The results suggested that the system does not achieve steady state as quickly as once expected. One hour is sufficient to achieve steady-state assuming the given constants are accurate, but further research should be done to validate the accuracy of the values. Certain deviations of the given constants from the predicted values resulted in steady-state time significantly longer than one hour. Additionally, Zero Net Flux was shown to be an accurate method of determining the concentration of glucose. However, if the system does not achieve steady-state, the concentration predicted by Zero Net Flux would not necessarily be accurate, so ensuring the presence of steady-state after one hour is critical.